Journal: Cancer Cell International

Article Title: NSC632839 suppresses esophageal squamous cell carcinoma cell proliferation in vitro by triggering spindle assembly checkpoint-mediated mitotic arrest and CREB-Noxa-dependent apoptosis

doi: 10.1186/s12935-025-03831-w

Figure Lengend Snippet: NSC632839 causes cells arrest at M phase. ( A , B ) Cell cycle was analyzed by flow cytometry. NSC632839 treatment led to a concentration-dependent G2-M phase arrests. DMSO was used as control. Representative histograms for DMSO and NSC632839 were shown ( A ). Statistical analysis was shown in panel B. ( C , D ) Immunofluorescence microscopy of p-H3 expression. Cells were incubated with DMSO or NSC632839 and stained with phospho-histone H3 antibodies (red). DAPI (blue) was used to stain the nucleus. The DAPI and p-H3 staining were merged. Representative images were shown in panel C. Scale bar = 50 μm. Statistical analysis of p-H3-positive (mitotic) cells were shown in panel D. ( E ) The expression of p-H3 (Ser10) and H3 were evaluated by immunoblot assay. ( F ) NSC632839 disrupts the mitotic spindle organization. Cells were incubated with DMSO or NSC632839 and stained with anti-α-tubulin antibodies (green). DAPI (blue) was used to detect DNA. Cells were analyzed and representative immunofluorescence images are shown. Scale bar = 15 μm. ( G ) The expression of p-Aurora A/B/C, Aurora A/B, p-PLK1 and PLK1 were detected by immunoblot assay. ( H ) Nuclear morphologies of cells were stained with α-tubulin (green) antibody and DAPI (blue). Scale bar = 50 μm

Article Snippet: Primary antibodies against cleaved CASP3, Bcl-xl, phosphorylated-Histone H3 (Ser10) (p-H3), CIAP1, cleaved PARP, ATF4, LC3, FOXO3a, PARP, CIAP2, Bax, Puma, XIAP (Cell Signaling Technology, Inc, Boston, MA), p53, Ub, Noxa, and c-Myc (Santa Cruz Biotechnology, Santa Cruz, CA), HIF-1α (Abgent, Shanghai, China), phosphorylated-p53(Ser15), Histone H3 (H3), Capase3, p-Aurora and α-Tubulin (Beyotime, China).

Techniques: Flow Cytometry, Concentration Assay, Control, Immunofluorescence, Microscopy, Expressing, Incubation, Staining, Western Blot

Journal: Frontiers in Pharmacology

Article Title: Xixin Decoction’s novel mechanism for alleviating Alzheimer’s disease cognitive dysfunction by modulating amyloid-β transport across the blood–brain barrier to reduce neuroinflammation

doi: 10.3389/fphar.2024.1508726

Figure Lengend Snippet: XXD Upregulates TREM2 Expression and Downregulates IBA1, TLR1, and TLR2 Expression, Inhibiting Microglial Activation; (A) and (E) Analysis of relative IBA1 protein expression levels and average fluorescence intensity of IBA1 in the hippocampal CA1 region of mice; (B) and (F) Analysis of relative TLR1 protein expression levels and average fluorescence intensity of TLR1 in the hippocampal CA1 region of mice; (C) and (G) Analysis of relative TLR2 protein expression levels and average fluorescence intensity of TLR2 in the hippocampal CA1 region of mice; (D) and (H) Analysis of relative TREM2 protein expression levels and average fluorescence intensity of TREM2 in the hippocampal CA1 region of mice; (n = 6); Scale bar: 20μm; images captured at ×400 magnification; ** p < 0.01, * p < 0.05.

Article Snippet: The antibody against IBA1 (8525-1904) was purchased from Cell Signaling Technology (CST, United States).

Techniques: Expressing, Activation Assay, Fluorescence

Journal: Frontiers in Pharmacology

Article Title: Xixin Decoction’s novel mechanism for alleviating Alzheimer’s disease cognitive dysfunction by modulating amyloid-β transport across the blood–brain barrier to reduce neuroinflammation

doi: 10.3389/fphar.2024.1508726

Figure Lengend Snippet: XXD reduces the levels of inflammation-related molecules and cytokines in the hippocampus: (A) Comparison of relative expression levels of CMPK2 protein in the hippocampus of different groups of mice; (B) Comparison of NLRP3 protein levels in the hippocampus of different groups of mice; (C) Comparison of IBA1 protein levels in the hippocampus of different groups of mice; (D) Comparison of COX-2 protein levels in the hippocampus of different groups of mice; (E) Comparison of NF-κB p65 protein levels in the hippocampus of different groups of mice; (F) Comparison of IL-1β protein levels in the hippocampus of different groups of mice; (G) Comparison of TNF-α protein levels in the hippocampus of different groups of mice. (n = 6); ** p < 0.01, * p < 0.05.

Article Snippet: The antibody against IBA1 (8525-1904) was purchased from Cell Signaling Technology (CST, United States).

Techniques: Comparison, Expressing